TRIPOLYPHOSPHATE AND TRIMETAPHOSPHATE IN YEAST EXTRACTS

Tripolyphosphate and trimetaphosphate in yeast extracts.

Electrophoretic Analysis of Yeast Extracts

Enzymatic Activity Assays in Yeast Cell Extracts.

Saccharomyces cerevisiae (S. cerevisiae) (commonly known as baker's yeast) is a model organism that has a similar upstream base excision repair (BER) pathway for the repair of methylated bases as that in mammalian cells, and it is very easy to maintain in the laboratory environment. Here, we described a method to prepare cell extracts from yeast to investigate their enzymatic activities. This p...

Chromatin assembly in yeast cell-free extracts.

A simple method for preparing chromatin assembly extracts has not been available for budding yeast. Here I describe such a method in detail. The assembly extract, a crude 100,000g supernatant, is prepared from cells disrupted in a manual or motorized grinder while they are frozen. The core histones and all soluble protein factors required for chromatin assembly under physiological conditions ar...

Spontaneous spatiotemporal organization in yeast extracts.

Stirred cytoplasmic extracts of the yeast Saccharomyces uvarum can, on addition of trehalose, display oscillations in the metabolite concentrations. Unstirred extracts may also develop spatiotemporal structures expressed in the metabolite concentrations. In this paper we present evidence of the existence of such patterns. The latter were recorded using the optical absorption of NADH in an annul...